Journal article
Characterization of Isolates of Salmonella enterica Serovar Stanley, a Serovar Endemic to Asia and Associated with Travel
Division of Microbiology and Risk Assessment, National Food Institute, Technical University of Denmark1
National Food Institute, Technical University of Denmark2
WHO Collaborating Centre for Reference and Research on Salmonella, France3
Thailand Ministry of Public Health4
Statens Serum Institut5
Salmonella enterica serovar Stanley (S. Stanley) is a common serovar in Southeast Asia and was the second most common serovar implicated in human salmonellosis in Thailand in the years 2002 to 2007. In contrast, this serovar is relatively uncommon in Europe. The objective of this study was to characterize a collection of S.
Stanley strains isolated from Thai (n = 62), Danish (n = 39), and French (n = 24) patients to gain a broader understanding of the genetic diversity, population dynamics, and susceptibility to antimicrobials. All isolates were characterized by pulsed-field gel electrophoresis and antimicrobial susceptibility testing.
The molecular mechanisms of resistance to extended-spectrum cephalosporins and plasmid-mediated resistance to quinolones were characterized by PCR and sequencing. Plasmid profiling, replicon typing, and microarray analysis were used to characterize the genetic mechanisms of antimicrobial resistance in 10 extended-spectrum cephalosporinase-producing isolates.
Considerable genetic diversity was observed among the isolates characterized with 91 unique XbaI pulsed-field gel electrophoresis (PFGE) patterns, including 17 distinct clusters consisting of two to seven indistinguishable isolates. We found some of the S. Stanley isolates isolated from patients in Europe were acquired during travel to Southeast Asia, including Thailand.
The presence of multiple plasmid lineages carrying the extended-spectrum cephalosporinase-encoding blaCMY-2 gene in S. Stanley isolates from the central part of Thailand was confirmed. Our results emphasize that Thai authorities, as well as authorities in other countries lacking prudent use of antimicrobials, should improve the ongoing efforts to regulate antimicrobial use in agriculture and in clinical settings to limit the spread of multidrug-resistant Salmonella isolates and plasmids among humans and pigs in Thailand and abroad.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2012 |
Pages: | 709-720 |
ISSN: | 1098660x , 00951137 and 1070633x |
Types: | Journal article |
DOI: | 10.1128/JCM.05943-11 |
ORCIDs: | Hendriksen, Rene S. , Bortolaia, Valeria , Svendsen, Christina Aaby and Aarestrup, Frank Møller |
Adolescent Adult Aged Aged, 80 and over Anti-Bacterial Agents Child Child, Preschool Denmark Electrophoresis, Gel, Pulsed-Field Female France Genetic Variation Genotype Humans Infant Infant, Newborn Male Microbial Sensitivity Tests Middle Aged Molecular Typing Plasmids Polymerase Chain Reaction Salmonella Infections Salmonella enterica Sequence Analysis, DNA Serotyping Thailand Travel Young Adult