Journal article
Time-dependent changes in protein expression in rainbow trout muscle following hypoxia
Section for Aquatic Protein Biochemistry, National Institute of Aquatic Resources, Technical University of Denmark1
National Food Institute, Technical University of Denmark2
Division of Industrial Food Research, National Food Institute, Technical University of Denmark3
National Institute of Aquatic Resources, Technical University of Denmark4
Section for Aquaculture, National Institute of Aquatic Resources, Technical University of Denmark5
Technical University of Denmark6
Adaptation to hypoxia is a complex process, and individual proteins will be up- or down-regulated in order to address the main challenges at any given time. To investigate the dynamics of the adaptation, rainbow trout (Oncorhynchus mykiss) was exposed to 30% of normal oxygen tension for 1, 2, 5 and 24h respectively, after which muscle samples were taken.
The successful investigation of numerous proteins in a single study was achieved by selectively separating the sarcoplasmic proteins using 2-DE. In total 46 protein spots were identified as changing in abundance in response to hypoxia using one-way ANOVA and multivariate data analysis. Proteins of interest were subsequently identified by MS/MS following tryptic digestion.
The observed regulation following hypoxia in skeletal muscle was determined to be time specific, as only a limited number of proteins were regulated in response to more than one time point. The cellular response to hypoxia included regulation of proteins involved in maintaining iron homeostasis, energy levels and muscle structure.
In conclusion, this proteome-based study presents a comprehensive investigation of the expression profiles of numerous proteins at four different time points. This increases our understanding of timed changes in protein expression in rainbow trout muscle following hypoxia.
Language: | English |
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Year: | 2012 |
Pages: | 2342-2351 |
ISSN: | 18767737 and 18743919 |
Types: | Journal article |
DOI: | 10.1016/j.jprot.2012.02.010 |
ORCIDs: | Wulff, Tune , Jokumsen, Alfred and Jessen, Flemming |