Journal article
Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap
A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing.
A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR).
We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC).
Language: | English |
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Publisher: | Taylor & Francis Group |
Year: | 2011 |
Pages: | 166-174 |
ISSN: | 15322297 and 10826068 |
Types: | Journal article |
DOI: | 10.1080/10826068.2011.547366 |
ORCIDs: | Hoorfar, Jeffrey |