Journal article
High throughput label-free platform for statistical bio-molecular sensing
Nanoprobes Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Academia Sinica Taiwan4
Surface Engineering Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
Sensors are crucial in many daily operations including security, environmental control, human diagnostics and patient monitoring. Screening and online monitoring require reliable and high-throughput sensing. We report on the demonstration of a high-throughput label-free sensor platform utilizing cantilever based sensors.
These sensors have often been acclaimed to facilitate highly parallelized operation. Unfortunately, so far no concept has been presented which offers large datasets as well as easy liquid sample handling. We use optics and mechanics from a DVD player to handle liquid samples and to read-out cantilever deflection and resonant frequency.
Also, surface roughness is measured. When combined with cantilever deflection the roughness is discovered to hold valuable additional information on specific and unspecific binding events. In a few minutes, 30 liquid samples can be analyzed in parallel, each by 24 cantilever-based sensors. The approach was used to detect the binding of streptavidin and antibodies.
Language: | English |
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Publisher: | The Royal Society of Chemistry |
Year: | 2011 |
Pages: | 2411-2416 |
ISSN: | 14730189 and 14730197 |
Types: | Journal article |
DOI: | 10.1039/c1lc20116f |
ORCIDs: | Keller, Stephan Sylvest , Bache, Michael , Jakobsen, Mogens Havsteen and Boisen, Anja |