Journal article
Microfluidic dissolved oxygen gradient generator biochip as a useful tool in bacterial biofilm studies
Polymeric Enabling Microsystems Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Bioanalytics Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark6
Department of Systems Biology, Technical University of Denmark7
Polymer Microsystems for Electrophysiology Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark8
Fluidic Array Systems and Technology Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark9
A microfluidic chip for generation of gradients of dissolved oxygen was designed, fabricated and tested. The novel way of active oxygen depletion through a gas permeable membrane was applied. Numerical simulations for generation of O-2 gradients were correlated with measured oxygen concentrations. The developed microsystem was used to study growth patterns of the bacterium Pseudomonas aeruginosa in medium with different oxygen concentrations.
The results showed that attachment of Pseudomonas aeruginosa to the substrate changed with oxygen concentration. This demonstrates that the device can be used for studies requiring controlled oxygen levels and for future studies of microaerobic and anaerobic conditions.
| Language: | English |
|---|---|
| Publisher: | The Royal Society of Chemistry |
| Year: | 2010 |
| Pages: | 2162-2169 |
| ISSN: | 14730189 and 14730197 |
| Types: | Journal article |
| DOI: | 10.1039/c003558k |
| ORCIDs: | Emnéus, Jenny , Molin, Søren , Taboryski, Rafael Jozef , Sternberg, Claus and Dufva, Martin |
