Journal article
Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
Delft University of Technology1
Swiss Federal Institute of Technology Zurich2
Laboratoire Bordelais de Recherche en Informatique3
University of Cambridge4
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark5
Department of Systems Biology, Technical University of Denmark6
Chalmers University of Technology7
VTT Technical Research Centre of Finland Ltd.8
Vrije Universiteit Amsterdam9
University of Stuttgart10
Bogazici University11
...and 1 moreThe field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals.
With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques.
Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.
Language: | English |
---|---|
Publisher: | Nature Publishing Group |
Year: | 2010 |
Pages: | 145 |
ISSN: | 20411723 |
Types: | Journal article |
DOI: | 10.1038/ncomms1150 |
ORCIDs: | Workman, Christopher |