Journal article
Blu-ray based optomagnetic aptasensor for detection of small molecules
Columbia University1
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
University of the Basque Country4
Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark5
Yonsei University6
Department of Applied Mathematics and Computer Science, Technical University of Denmark7
Cognitive Systems, Department of Applied Mathematics and Computer Science, Technical University of Denmark8
CIC nanoGUNE Consolider9
Magnetic Systems, Department of Micro- and Nanotechnology, Technical University of Denmark10
...and 0 moreThis paper describes an aptamer-based optomagnetic biosensor for detection of a small molecule based on target binding-induced inhibition of magnetic nanoparticle (MNP) clustering. For the detection of a target small molecule, two mutually exclusive binding reactions (aptamer-target binding and aptamer-DNA linker hybridization) are designed.
An aptamer specific to the target and a DNA linker complementary to a part of the aptamer sequence are immobilized onto separate MNPs. Hybridization of the DNA linker and the aptamer induces formation of MNP clusters. The target-to-aptamer binding on MNPs prior to the addition of linker-functionalized MNPs significantly hinders the hybridization reaction, thus reducing the degree of MNP clustering.
The clustering state, which is thus related to the target concentration, is then quantitatively determined by an optomagnetic readout technique that provides the hydrodynamic size distribution of MNPs and their clusters. A commercial Blu-ray optical pickup unit is used for optical signal acquisition, which enables the establishment of a low-cost and miniaturized biosensing platform.
Experimental results show that the degree of MNP clustering correlates well with the concentration of a target small molecule, adenosine triphosphate (ATP) in this work, in the range between 10µM and 10mM. This successful proof-of-concept indicates that our optomagnetic aptasensor can be further developed as a low-cost biosensing platform for detection of small molecule biomarkers in an out-of-lab setting.
Language: | English |
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Year: | 2016 |
Pages: | 396-403 |
ISSN: | 18734235 and 09565663 |
Types: | Journal article |
DOI: | 10.1016/j.bios.2015.08.062 |
ORCIDs: | Alstrøm, Tommy Sonne , Boisen, Anja and Hansen, Mikkel Fougt |