Journal article
Ex vivo intestinal perfusion model for investigating mucoadhesion of microcontainers
Department of Health Technology, Technical University of Denmark1
Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark2
Nanoprobes, Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark3
University of Copenhagen4
National Centre for Nano Fabrication and Characterization, Technical University of Denmark5
Nanofabrication, National Centre for Nano Fabrication and Characterization, Technical University of Denmark6
Biomaterial Microsystems, Nanofabrication, National Centre for Nano Fabrication and Characterization, Technical University of Denmark7
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark8
Micro fabricated delivery systems have shown promise in increasing oral bioavailability of drugs. Micrometer-sized polymeric devices (microcontainers) have the potential to facilitate unidirectional drug release directly into the intestinal mucosa whereby, drug absorption can be enhanced. The aim of this study was to develop an ex vivo model to investigate mucosal adhesion and orientation of microcontainers.
Furthermore, to investigate how microcontainers with varying height, shape and material behave in regards to mucoadhesion and orientation. Microcontainers were placed at the top of an inclined piece of porcine small intestine. The tissue was perfused with biorelevant medium followed by microscopic examination to observe the orientation and amount of microcontainers on the tissue.
The mucoadhesion of the microcontainers were evaluated based on the observed position on the tissue after being exposed to flow. When comparing the varying types of microcontainers, good adhesion was in general observed since most of the microcontainers were located in the beginning of the intestine.
Microcontainers fabricated from the epoxy-based photoresist SU-8 had a slightly better adherence than those fabricated from poly-ɛ-caprolactone (PCL). The orientation of the microcontainers appeare to be dictated mainly by the height. In general, the model showed promising results in evaluating mucoadhesion and orientation.
Language: | English |
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Year: | 2019 |
Pages: | 118658 |
ISSN: | 18733476 and 03785173 |
Types: | Journal article |
DOI: | 10.1016/j.ijpharm.2019.118658 |
ORCIDs: | Petersen, Ritika Singh , Thamdrup, Lasse Højlund Eklund , Keller, Stephan Sylvest , Nielsen, Line Hagner , Boisen, Anja and 0000-0003-3020-8892 |