Journal article
A biochemically structured model for ethanol fermentation by Kluyveromyces marxianus: A batch fermentation and kinetic study
Computer Aided Process Engineering Center, Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Department of Chemical and Biochemical Engineering, Technical University of Denmark2
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3
Department of Systems Biology, Technical University of Denmark4
University of Calabria5
CHEC Research Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark6
Anaerobic batch fermentations of ricotta cheese whey (i.e. containing lactose) were performed under different operating conditions. Ethanol concentrations of ca. 22gL−1 were found from whey containing ca. 44gL−1 lactose, which corresponded to up to 95% of the theoretical ethanol yield within 15h. The experimental data could be explained by means of a simple knowledge-driven biochemically structured model that was built on bioenergetics principles applied to the metabolic pathways through which lactose is converted into major products.
Use of the model showed that the observed concentrations of ethanol, lactose, biomass and glycerol during batch fermentation could be described within a ca. 6% deviation, as could the yield coefficients for biomass and ethanol produced on lactose. The model structure confirmed that the thermodynamics considerations on the stoichiometry of the system constrain the metabolic coefficients within a physically meaningful range thereby providing valuable and reliable insight into fermentation processes.
Language: | English |
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Year: | 2011 |
Pages: | 7513-7520 |
ISSN: | 18732976 and 09608524 |
Types: | Journal article |
DOI: | 10.1016/j.biortech.2011.05.014 |
ORCIDs: | Hobley, Timothy John , Villadsen, John and Sin, Gürkan |
Cheese Ethanol Fermentation Kinetics Kluyveromyces Models, Chemical