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Journal article

Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers

By Crouzier, Lucile1; Dubois, Camille1; Edwards, Stacey L1; Lauridsen, Lasse H2; Wengel, Jesper3; Veedu, Rakesh N.1

Edited by Antopolsky, Maxim

From

University of Queensland1

Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2

University of Southern Denmark3

We found that SuperScript® III Reverse Transcriptase is an efficient enzyme for the recognition of LNA nucleotides, making it a prime candidate to be used in de novo selection of LNA containing RNA aptamers.

Language: English
Publisher: Public Library of Science
Year: 2012
Pages: e35990
ISSN: 19326203
Types: Journal article
DOI: 10.1371/journal.pone.0035990
ORCIDs: Lauridsen, Lasse H
Keywords

Aptamers, Nucleotide Base Sequence DNA, Complementary Medicine Molecular Sequence Data Nucleotides Oligonucleotides Polymerase Chain Reaction Q R RNA-Directed DNA Polymerase Reverse Transcription Ribonucleases Science Templates, Genetic locked nucleic acid

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