Journal article
Genomic characterization of Flavobacterium psychrophilum serotypes and development of a multiplex PCR-based serotyping scheme
Université Paris-Saclay1
Kindai University2
INRAE3
National Veterinary Institute, Technical University of Denmark4
Bacteriology & Parasitology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark5
Fish Diseases, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark6
Université d'Évry Val-d'Essonne7
Åbo Akademi University8
Flavobacterium psychrophilum is a devastating bacterial pathogen of salmonids reared in freshwater worldwide. So far, serological diversity between isolates has been described but the underlying molecular factors remain unknown. By combining complete genome sequence analysis and the serotyping method proposed by Lorenzen and Olesen (1997) for a set of 34 strains, we identified key molecular determinants of the serotypes.
This knowledge allowed us to develop a robust multiplex PCR-based serotyping scheme, which was applied to 244 bacterial isolates. The results revealed a striking association between PCR-serotype and fish host species and illustrate the use of this approach as a simple and cost-effective method for the determination of F. psychrophilum serogroups.
PCR-based serotyping could be a useful tool in a range of applications such as disease surveillance, selection of salmonids for bacterial coldwater disease resistance and future vaccine formulation.
Language: | English |
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Publisher: | Frontiers Media S.A. |
Year: | 2017 |
Pages: | 1752 |
ISSN: | 1664302x |
Types: | Journal article |
DOI: | 10.3389/fmicb.2017.01752 |
ORCIDs: | Dalsgaard, Inger and Madsen, Lone |
Fish-pathogenic bacteria Flavobacterium psychrophilum Genomics SDG 3 - Good Health and Well-being Salmonid aquaculture Serotype mPCR
Microbiology QR1-502 fish-pathogenic bacteria genomics salmonid aquaculture serotype