Journal article
The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth
Institute of Molecular Biology, Department of Plant Physiology, Øster Farimagsgade 2A, 1353K Copenhagen, Denmark.1
The lue1 mutant was previously isolated in a bio-imaging screen for Arabidopsis mutants exhibiting inappropriate regulation of an AtGA20ox1 promoter-luciferase reporter fusion. Here we show that lue1 is allelic to fra2, bot1 and erh3, and encodes a truncated katanin-like microtubule-severing protein (AtKSS).
Complementation of lue1 with the wild-type AtKSS gene restored both wild-type stature and luciferase reporter levels. Hormonal responses of lue1 to ethylene and gibberellins revealed inappropriate cortical microtubule reorientation during cell growth. Moreover, a fusion between the AtKSS protein and GFP decorated cortical microtubules.
A yeast two-hybrid screen with AtKSS as the bait identified proteins related to those involved in microtubule processing, including a katanin p80 subunit and a kinesin ortholog. These results indicate that AtKSS is involved in microtubule dynamics in response to plant hormones.
| Language: | English |
|---|---|
| Year: | 2003 |
| Pages: | 791-801 |
| ISSN: | 02693518 , 00219533 and 14779137 |
| Types: | Journal article |
| DOI: | 10.1242/jcs.00274 |
| ORCIDs: | Mundy, John |
Adenosine Triphosphatases Amino Acid Sequence Arabidopsis Arabidopsis Proteins Cell Division Ethylenes Gene Expression Regulation, Plant Genes, Reporter Gibberellins Glucuronidase KTN1 protein, Arabidopsis Katanin Microfibrils Microscopy, Polarization Microtubules Molecular Sequence Data Mutation Phenotype Plant Growth Regulators Plant Roots RNA, Messenger Sequence Homology, Amino Acid ethylene
