Journal article
Occurrence of different trichothecenes and deoxynivalenol-3-β-D-glucoside in naturally and artificially contaminated Danish cereal grains and whole maize plants
National Food Institute, Technical University of Denmark1
Division of Food Chemistry, National Food Institute, Technical University of Denmark2
Department of Systems Biology, Technical University of Denmark3
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark4
Aarhus University5
Knowledge Centre for Agriculture6
Fusarium mycotoxins such as deoxynivalenol (DON) can occur in cereals conjugated to glucose and probably also to other sugars. These conjugates, which are often referred to as "masked mycotoxins", will not be detected with routine analytical techniques. Furthermore, it is suspected that the parent toxin may again be released after hydrolysis in the digestive tracts of animals and humans.
Today, our knowledge of the occurrence of these compounds in cereal grains is limited. In this paper, a LC-MS/MS method for the simultaneous determination of DON, deoxynivalenol-3-β-D-glucoside (DON-3-glucoside), 3 acetyl-DON, nivalenol, fusarenon-X, diacetoxyscirpenol, HT-2 toxin, and T-2 toxin in naturally (n = 48) and artificially (n = 30) contaminated cereal grains (wheat, barley, oat, rye triticale) is reported.
The method has also been applied to whole fresh maize plant intended for production of maize silage (n = 10). The samples were collected from the harvest years 2006–2010, The results show that DON-3-glucoside and DON co-occurred in cereal grains and, especially in several of the highly contaminated samples, the concentration of the glucoside can be relatively high, corresponding to over 37 % of the DON concentration.
The DON-3-glucoside levels in both the naturally and in the artificially grain inoculated with Fusarium were second only to DON, and were generally higher than those of the other tested trichothecenes, which were found at low concentrations in most samples, in many cases even below the detection limit of the method.
This argues for the importance of taking DON-3-glucoside into account in the ongoing discussion within the European Community concerning exposure re-evaluations for setting changed values for the tolerable intake for DON. Our results indicate that, in the naturally contaminated grains and in the Fusarium infested cereal grains (winter and spring wheat, oat, triticale), the concentration level of DON-3-glucoside is positively correlated to the DON content.
When the DON concentration is high, then the content of DON-3-glucoside will most probably also be high and vice versa.
| Language: | English |
|---|---|
| Publisher: | Springer-Verlag |
| Year: | 2012 |
| Pages: | 181-190 |
| ISSN: | 18671632 and 01787888 |
| Types: | Journal article |
| DOI: | 10.1007/s12550-012-0133-z |
| ORCIDs: | Rasmussen, P. H. and Nielsen, Kristian Fog |
Avena Chemistry/Food Science, general Chromatography, Liquid Denmark Deoxynivalenol (DON) Edible Grain Food Contamination Food Microbiology Glucosides Grain species Hordeum LC-MS/MS Life Sciences Masked mycotoxin (DON-3-<beta>-D-glucoside) Masked mycotoxin (DON-3-β-d-glucoside) Mass Spectrometry Medical Microbiology Medicine/Public Health, general Microbiology Secale Toxins Trichothecenes Triticum Zea mays deoxynivalenol-3-glucoside
