Journal article
Molecular characterization of SAT 2 foot-and-mouth disease virus from post-outbreak slaughtered animals: implications for disease control in Uganda
In Uganda, limiting the extent of foot-and-mouth disease (FMD) spread during outbreaks involves short term measures such as ring vaccination and restrictions to the movement of livestock and their products to and from the affected areas. In this study, the presence of FMD virus RNA was investigated in cattle samples, three months after FMD quarantine measures had been lifted in the area in 2004 following an outbreak.
Oropharyngeal tissue samples were obtained from 12 cattle slaughtered in a small town abattoir of Kiboga. FMD virus RNA was detected by diagnostic RT- PCR in 9 of the 12 tissue samples. Part of the coding region for the capsid protein VP1 was amplified and sequenced. All samples were identified as belonging to the SAT 2 serotype.
The implications for FMD control of both virus introductions into Uganda and the presence of carrier animals following outbreaks are discussed.
| Language: | English |
|---|---|
| Publisher: | Cambridge University Press |
| Year: | 2010 |
| Pages: | 1204-1210 |
| ISSN: | 14694409 and 09502688 |
| Types: | Journal article |
| DOI: | 10.1017/S0950268809991427 |
| ORCIDs: | 0000-0001-5757-3131 and Belsham, Graham |
Animals Base Composition Base Sequence Capsid Proteins Cattle Disease Outbreaks FMD control Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Molecular Sequence Data Phylogeny Quarantine RNA, Viral Reverse Transcriptase Polymerase Chain Reaction SAT 2 Sequence Alignment Uganda VP1 protein, Foot-and-mouth disease virus sequence divergence
