Conference paper
New producers and new 11-, 19-, and 20-residue peptaibiotics: suzukacillins B and C
Fraunhofer-Gesellschaft1
Department of Systems Biology, Technical University of Denmark2
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark3
Justus Liebig University Giessen4
University of Vienna5
Section for Building Physics and Services, Department of Civil Engineering, Technical University of Denmark6
In the course of a project aimed to assess the significance of antibiotics for the producing organism(s) in the natural habitat, we screened a specimen of the fungicolous fungus Hypocrea phellinicola growing on its natural host Phellinus ferruginosus1. Using a peptaibiomics approach2,3, we detected 19- and 20-residue peptide sequences by (U)HPLC/HR-ESI-QqTOF-MS.
Structures of peptaibiotics found were independently confirmed by analyzing the peptaibiome of an agar plate culture of H. phellinicola CBS 119283 (ex-type) grown under laboratory conditions. Notably, H. phellinicola could be identified as a potent producer of 18-, 19-, (culture) and 20-residue (specimen) peptaibiotics of the suzukacillin-type4.
Minor components of the 19-residue peptaibols, herein named suzukacillins C, are assumed to carry a C-terminal residue tentatively assigned as tyrosinol (Tyrol). In addition, the previously isolated suzukacillin B4 was sequenced and shown to be a microheterogeneous mixture of 11-residue peptaibols.
| Language: | English |
|---|---|
| Year: | 2012 |
| Proceedings: | 32nd EPS Meeting |
| ISSN: | 10991387 and 10752617 |
| Types: | Conference paper |
| ORCIDs: | Thrane, Ulf and Nielsen, Kristian Fog |
