Journal article
Method Enabling Gene Expression Studies of Pathogens in a Complex Food Matrix
In
Applied and Environmental Microbiology
—
2011, Volume 77, Issue 23, pp. 8456-8458
Abstract
We describe a simple method for stabilizing and extracting high-quality prokaryotic RNA from meat. Heat and salt stress of Escherichia coli and Salmonella spp. in minced meat reproducibly induced dnaK and otsB expression, respectively, as observed by quantitative reverse transcription-PCR (>5-fold relative changes).
Thus, the method is applicable in studies of bacterial gene expression in a meat matrix.
| Language: | English |
|---|---|
| Publisher: | American Society for Microbiology |
| Year: | 2011 |
| Pages: | 8456-8458 |
| ISSN: | 10985336 and 00992240 |
| Types: | Journal article |
| DOI: | 10.1128/AEM.05471-11 |
| ORCIDs: | 0000-0002-8350-5631 and Aabo, Søren |
