Journal article
Investigations on antibody binding to a micro-cantilever coated with a BAM pesticide residue
Department of Micro- and Nanotechnology, Technical University of Denmark1
Geological Survey of Denmark and Greenland2
Surface Engineering Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark3
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout.
The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system. © 2011 Bache et al.
Language: | English |
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Publisher: | Springer |
Year: | 2011 |
Pages: | 1-5 |
ISSN: | 1556276x and 19317573 |
Types: | Journal article |
DOI: | 10.1186/1556-276X-6-386 |
ORCIDs: | Bache, Michael , Taboryski, Rafael Jozef , Schmid, Silvan and Jakobsen, Mogens Havsteen |