Journal article
Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients
Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated.
The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed.
Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype.
The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.
Language: | English |
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Year: | 2011 |
Pages: | 263-274 |
ISSN: | 16000463 , 09034641 , 0903465x and 16005503 |
Types: | Journal article |
DOI: | 10.1111/j.1600-0463.2011.02726.x |
ORCIDs: | Molin, Søren , 0000-0002-1347-725X and 0000-0001-5648-4858 |
AlgU protein, Pseudomonas aeruginosa Alginates Bacterial Proteins Biofilms Chronic Disease Cystic Fibrosis Denmark Gene Expression Profiling Genes, Bacterial Genotype Glucuronic Acid Hexuronic Acids Humans LasR protein, Pseudomonas aeruginosa Lung Diseases MucA protein, Pseudomonas Mutation Oligonucleotide Array Sequence Analysis Phenotype Pseudomonas Infections Pseudomonas aeruginosa Quorum Sensing RhlR protein, Pseudomonas aeruginosa Sigma Factor Trans-Activators