Journal article
Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering
Polytechnic University of Turin1
Nanoprobes, Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark2
Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark3
Department of Health Technology, Technical University of Denmark4
Bacterial Cell Factory Optimization, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark7
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark8
Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites.
By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.
Language: | English |
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Publisher: | The Royal Society of Chemistry |
Year: | 2019 |
Pages: | 1600-1607 |
ISSN: | 13645528 and 00032654 |
Types: | Journal article |
DOI: | 10.1039/C8AN02128G |
ORCIDs: | Ilchenko, Oleksii , Jendresen, Christian Bille , Nielsen, Alex Toftgaard , Zor, Kinga , Boisen, Anja , 0000-0002-3299-8608 , 0000-0001-5374-1679 and 0000-0002-3789-4816 |