Journal article
Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures
A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
Language: | English |
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Year: | 1997 |
Pages: | 3698-3702 |
ISSN: | 10985336 and 00992240 |
Types: | Journal article |
DOI: | 10.1128/aem.63.9.3698-3702.1997 |
ORCIDs: | Molin, Søren |