Journal article
gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication
In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P-luxI have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins.
Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations.
This green fluorescent AHL sensor provides a state-of-the art tool for studies of communication between the individuals present in mixed bacterial communities.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 2001 |
Pages: | 575-585 |
ISSN: | 10985336 and 00992240 |
Types: | Journal article |
DOI: | 10.1128/AEM.67.2.575-585.2001 |
ORCIDs: | Christensen, Bjarke Bak and Molin, Søren |
4-Butyrolactone Bacterial Proteins Biosensing Techniques Chemotaxis Gene Expression Regulation, Bacterial Gram-Negative Bacteria Green Fluorescent Proteins Image Processing, Computer-Assisted Luminescent Proteins LuxI protein, Bacteria LuxR autoinducer binding proteins Microscopy, Confocal N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone Plasmids Promoter Regions, Genetic Repressor Proteins Signal Transduction Trans-Activators Transcription Factors homoserine lactone