Conference paper · Journal article
Characterization of the AN6448 cluster in Aspergillus nidulans
Department of Systems Biology, Technical University of Denmark1
Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark2
Organic Chemistry, Department of Chemistry, Technical University of Denmark3
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark4
Department of Chemistry, Technical University of Denmark5
With the aim of mapping the polyketome of A. nidulans we have made a library of strains, which individually overexpress PKS genes from an ectopic locus. A screen of this collection on different media demonstrated that AN6448 leads to production of 3-MOA. An inspection of the DNA sequence surrounding this gene uncovered a putative gene cluster including a gene, AN6446, with homology to transcription factors.
Based on this observation, we decided to overexpress AN6446. A qRT-PCR analysis of this strain was used to delineate the borders of the gene cluster as well as to stimulate formation of cichorine and a number of new products, from the gene cluster. Subsequent deletion of all genes allowed several steps in the biosynthetic pathway in this cluster to be clarified.
Language: | English |
---|---|
Year: | 2012 |
Pages: | 1113-1113 |
Proceedings: | International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF |
ISSN: | 14390221 and 00320943 |
Types: | Conference paper and Journal article |
DOI: | 10.1055/s-0032-1320546 |
ORCIDs: | Nielsen, Jakob Blæsbjerg , Gotfredsen, Charlotte Held , Larsen, T. O. and Mortensen, Uffe Hasbro |