Journal article
Microbial production of astilbin, a bioactive rhamnosylated flavanonol, from taxifolin
Flavonoids are plant-based polyphenolic biomolecules with a wide range of biological activities. Glycosylated flavonoids have drawn special attention in the industries as it improves solubility, stability, and bioactivity. Herein, we report the production of astilbin (ATN) from taxifolin (TFN) in genetically-engineered Escherichia coli BL21(DE3).
The exogenously supplied TFN was converted to ATN by 3-O-rhamnosylation utilizing the endogeneous TDP-l-rhamnose in presence of UDP-glycosyltransferase (ArGT3, Gene Bank accession number: At1g30530) from Arabidopsis thaliana. Upon improving the intracellular TDP-l-rhamnose pool by knocking out the chromosomal glucose phosphate isomerase (pgi) and d-glucose-6-phosphate dehydrogenase ( zwf) deletion along with the overexpression of rhamnose biosynthetic pathway increases the biotransformation product, ATN with total conversion of similar to 49.5 +/- 1.67% from 100 mu M of taxifolin.
In addition, the cytotoxic effect of taxifolin-3-O-rhamnoside on PANC-1 and A-549 cancer cell lines was assessed for establishing ATN as potent antitumor compound.
Language: | English |
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Publisher: | Springer Netherlands |
Year: | 2017 |
Pages: | 36 |
ISSN: | 15730972 and 09593993 |
Types: | Journal article |
DOI: | 10.1007/s11274-017-2208-7 |
ORCIDs: | Malla, Sailesh |
Biotransformation Cytotoxicity Escherichia coli Flavonoid Glycosylation SDG 3 - Good Health and Well-being
Antineoplastic Agents Applied Microbiology Arabidopsis Proteins Biochemistry, general Biosynthetic Pathways Biotechnology Cell Line, Tumor Cell Survival Chemistry Environmental Engineering/Biotechnology Flavonols Gene Knockout Techniques Genetic Engineering Glycosyltransferases Humans Microbiology Quercetin Rhamnose SC5 astilbin taxifolin