Journal article
Superparamagnetic bead interactions with functionalized surfaces characterized by an immunomicroarray
Department of Micro- and Nanotechnology, Technical University of Denmark1
Magnetic Systems Group, LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
LabChip Section, Department of Micro- and Nanotechnology, Technical University of Denmark3
Nano-Bio Integrated Systems Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark4
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Surface Engineering Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark7
Fluidic Array Systems and Technology Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark8
Magneto-resistive sensors capable of detecting superparamagnetic micro-/nano-sized beads are promising alternatives to standard diagnostic assays based on absorbance or fluorescence and streptavidin-functionalized beads are widely used as an integral part of these sensors. Here we have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO2 with or without surface modification SiO2 surface cleaning, immobilized substrate concentration and surface blocking conditions were optimized Polyethylene glycol-based surfaces with different end groups on the anchor molecule, 2,4,6-trichloro-1,3,5-triazine (TsT), were synthesized and compared with the standard (3-aminopropyl)triethoxysilane (APTS)/glutaraldehyde chemistry APTS/glutaraldehyde, directly linked TsT and bare H2O2-activated SiO2 performed better than polyethylene glycol-modified surfaces Two beads, Masterbeads and M-280 beads, were found to give superior results compared with other bead types.
Antibody/ antigen interactions, Illustrated by C-reactive protein, were best performed with Masterbeads The results provide important information concerning the surface binding properties of streptavidin-functionalized beads and the immunomicroarray can be used when optimizing the performance of bead-based biosensors.
Language: | English |
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Year: | 2010 |
Pages: | 3936-3946 |
ISSN: | 18787568 and 17427061 |
Types: | Journal article |
DOI: | 10.1016/j.actbio.2010.04.019 |
ORCIDs: | Hansen, Mikkel Fougt , Svendsen, Winnie Edith , Jakobsen, Mogens Havsteen and Dufva, Martin |