Journal article
Mutations in the Global Transcription Factor CRP/CAP: Insights from Experimental Evolution and Deep Sequencing
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Microbial Evolution and Synthetic Biology, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Institut de Cardiométabolisme et Nutrition4
Université Paris 55
The Escherichia coli cyclic AMP receptor protein (CRP or catabolite activator protein, CAP) provides a textbook example of bacterial transcriptional regulation and is one of the best studied transcription factors in biology. For almost five decades a large number of mutants, evolved in vivo or engineered in vitro, have shed light on the molecular structure and mechanism of CRP.
Here, we review previous work, providing an overview of studies describing the isolation of CRP mutants. Furthermore, we present new data on deep sequencing of different bacterial populations that have evolved under selective pressure that strongly favors mutations in the crp locus. Our new approach identifies more than 100 new CRP mutations and paves the way for a deeper understanding of this fascinating bacterial master regulator.
Language: | English |
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Publisher: | Research Network of Computational and Structural Biotechnology |
Year: | 2019 |
Pages: | 730-736 |
ISSN: | 20010370 |
Types: | Journal article |
DOI: | 10.1016/j.csbj.2019.05.009 |
ORCIDs: | Frendorf, Pernille Ott , Lauritsen, Ida and Nørholm, Morten H.H. |