Journal article
Molecular and functional assessment of multicellular cancer spheroids produced in double emulsions enabled by efficient airway resistance based selective surface treatment
Interdisciplinary Nanoscience Center - INANO-MBG, Gustav Wied 10, Interdisciplinary Nanoscience Center, Natural Sciences, Aarhus University
Department of Molecular Biology and Genetics, Natural Sciences, Aarhus University
Interdisciplinary Nanoscience Center - iNANO-MBG, Universitetsbyen, Interdisciplinary Nanoscience Center, Natural Sciences, Aarhus University
Department of Engineering, Technical Sciences, Aarhus University
Interdisciplinary Nanoscience Center - INANO-MBG, iNANO-huset, Interdisciplinary Nanoscience Center, Natural Sciences, Aarhus University
Chinese University of Hong Kong
Multicellular spheroids have garnered significant attention as an in vitro three-dimensional cancer model which can mimick the in vivo microenvironmental features. While microfluidics generated double emulsions have become a potential method to generate spheroids, challenges remain on the tedious procedures.
Enabled by a novel 'airway resistance' based selective surface treatment, this study presents an easy and facile generation of double emulsions for the initiation and cultivation of multicellular spheroids in a scaffold-free format. Combining with our previously developed DNA nanosensors, intestinal spheroids produced in the double emulsions have shown an elevated activities of an essential DNA modifying enzyme, the topoisomerase I.
The observed molecular and functional characteristics of spheroids produced in double emulsions are similar to the counterparts produced by the commercially available ultra-low attachment plates. However, the double emulsions excel for their improved uniformity, and the consistency of the results obtained by subsequent analysis of the spheroids.
The presented technique is expected to ease the burden of producing spheroids and to promote the spheroids model for cancer or stem cell study.
Language: | Undetermined |
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Year: | 2017 |
Pages: | 095014 |
ISSN: | 13616439 and 09601317 |
Types: | Journal article |
DOI: | 10.1088/1361-6439/aa7e92 |
ORCIDs: | 0000-0002-3484-3802 and Ho, Yi-Ping |