Journal article
Development and validation of an SPE HG-AAS method for determination of inorganic arsenic in samples of marine origin
The present paper describes a novel method for the quantitative determination of inorganic arsenic (iAs) in food and feed of marine origin. The samples were subjected to microwave-assisted extraction using diluted hydrochloric acid and hydrogen peroxide, which solubilised the analytes and oxidised arsenite (As(III)) to arsenate (As(V)).
Subsequently, a pH buffering of the sample extract at pH 6 enabled selective elution of As(V) from a strong anion exchange solid-phase extraction (SPE) cartridge. Hydride generation atomic absorption spectrometry (HG-AAS) was applied to quantify the concentration of iAs (sum of As(III) and As(V)) as the total arsenic (As) in the SPE eluate.
The results of the in-house validation showed that mean recoveries of 101-104% were achieved for samples spiked with iAs at 0.5, 1.0 and 1.5 mg·kg(-1), respectively. The limit of detection was 0.08 mg kg(-1), and the repeatability (RSD(r)) and intra-laboratory reproducibility (RSD(IR)) were less than 8% and 13%, respectively, for samples containing 0.2 to 1.5 mg kg(-1) iAs.
The trueness of the SPE HG-AAS method was verified by confirming results obtained by parallel analysis using high-performance liquid chromatography coupled to inductively coupled plasma mass spectrometry. It was demonstrated that the two sets of results were not significantly different (P <0.05). The SPE HG-AAS method was applied to 20 marine food and feed samples, and concentrations of up to 0.14 mg kg(-1) of iAs were detected.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2012 |
Pages: | 2825-2834 |
ISSN: | 16182650 , 16182642 and 09390065 |
Types: | Journal article |
DOI: | 10.1007/s00216-012-6006-7 |
ORCIDs: | Rasmussen, Rie Romme and Sloth, Jens Jørgen |
Analytical Chemistry Aquatic Organisms Arsenic Biochemistry, general Characterization and Evaluation of Materials Chemistry Chromatography, High Pressure Liquid Environmental Monitoring/Analysis Food Food Analysis Food Science Food and feed control Inorganic arsenic Laboratory Medicine Limit of Detection Marine samples Mass Spectrometry Microwave-assisted extraction Reproducibility of Results SPE HG-AAS Speciation