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Journal article

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

By Wales, Nathan1; Carøe, Christian2,3,4; Sandoval-Velasco, Marcela1; Gamba, Cristina1; Barnett, Ross1; Samaniego, Jose Alfredo1; Madrigal, Jazmin Ramos1; Orlando, Ludovic1; Gilbert, Thomas P.1

From

University of Copenhagen1

Department of Systems Biology, Technical University of Denmark2

Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark3

Metagenomics, Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark4

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens.

We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained

Language: English
Publisher: Future Science Ltd
Year: 2015
Pages: 368-371
ISSN: 19409818 and 07366205
Types: Journal article
DOI: 10.2144/000114364
ORCIDs: 0000-0003-0359-8450 , 0000-0001-5904-1198 , 0000-0002-1661-7991 , 0000-0003-3936-1850 and 0000-0002-5805-7195
Keywords

Biology (General) DNA DNA library preparation DNA, Single-Stranded Gene Library QH301-705.5 Sequence Analysis, DNA ancient DNA next-generation sequencing paleogenomics

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