Journal article
An automated and highly efficient method for counting and measuring fluorescent foci in rod-shaped bacteria : FLUORESCENT FOCI IN ROD-SHAPED BACTERIA
P>Direct measurements of cells from photo micrographs are becoming increasingly used when investigating the position and/or distribution of chromosomal loci in bacteria. In general, these measurements have been done manually, and without clear definition of how they are made. Here we present a procedure for standardizing the measurement of cell properties from phase contrast images.
Furthermore, we present a program using these standardized methods that can measure the intracellular positions of fluorescent foci in bacterial cells faster and with more precision than manual measurement.
Language: | English |
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Year: | 2010 |
Pages: | 194-199 |
ISSN: | 13652818 and 00222720 |
Types: | Journal article |
DOI: | 10.1111/j.1365-2818.2010.03374.x |