Journal article
Calibrated mitotic oscillator drives motile ciliogenesis
Institut de Biologie de l'École Normale Supérieure (IBENS), Paris Sciences et Lettres (PSL) Research University, Paris F-75005, France.1
CNRS, UMR 8197, Paris F-75005, France.2
INSERM, U1024, Paris F-75005, France.3
Sorbonne Universités, Université Pierre et Marie Curie (UPMC) Paris 06, INSERM, Centre de Recherche Saint-Antoine (CRSA), Paris F-75012, France.4
Institut de Biologie de l'École Normale Supérieure (IBENS), Paris Sciences et Lettres (PSL) Research University, Paris F-75005, France. alice.meunier@ens.fr.5
Cell division and differentiation depend on massive and rapid organelle remodeling. The mitotic oscillator, centered on the cyclin-dependent kinase 1-anaphase-promoting complex/cyclosome (CDK1-APC/C) axis, spatiotemporally coordinates this reorganization in dividing cells. Here we discovered that nondividing cells could also implement this mitotic clocklike regulatory circuit to orchestrate subcellular reorganization associated with differentiation.
We probed centriole amplification in differentiating mouse-brain multiciliated cells. These postmitotic progenitors fine-tuned mitotic oscillator activity to drive the orderly progression of centriole production, maturation, and motile ciliation while avoiding the mitosis commitment threshold. Insufficient CDK1 activity hindered differentiation, whereas excessive activity accelerated differentiation yet drove postmitotic progenitors into mitosis.
Thus, postmitotic cells can redeploy and calibrate the mitotic oscillator to uncouple cytoplasmic from nuclear dynamics for organelle remodeling associated with differentiation.
Language: | English |
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Publisher: | American Association for the Advancement of Science |
Year: | 2017 |
Pages: | 803-806 |
ISSN: | 10959203 and 00368075 |
Types: | Journal article |
DOI: | 10.1126/science.aan8311 |
ORCIDs: | Al Jord, Adel , Shihavuddin, Asm , Genovesio, Auguste , Karaiskou, Anthi , Sobczak-Thépot, Joëlle , Spassky, Nathalie and Meunier, Alice |