Journal article
Biotin Carboxyl Carrier Protein in Barley Chloroplast Membranes
Biotin localized in barley chloroplast lamellae is covalently bound to a single protein with an approximate molecular weight of 21000. It contains one mole of biotin per mole of protein and functions as a carboxyl carrier in the acetyl-CoA carboxylase reaction. The protein was obtained by solubilization of the lamellae in phenol/acetic acid/8 M urea.
Feeding barley seedlings with [14C]-biotin revealed that the vitamin is not degraded into respiratory substrates by the plant, but is specifically incorporated into biotin carboxyl carrier protein.
Language: | English |
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Year: | 1975 |
Pages: | 25-30 |
ISSN: | 00142956 and 14321033 |
Types: | Journal article |
DOI: | 10.1111/j.1432-1033.1975.tb04109.x |