Journal article
Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells : Antigen processing
University of Oxford1
NIHR Oxford Biomedical Research Centre2
Autonomous University of Barcelona3
Monash University4
University Hospital Vall d’Hebron5
Los Alamos National Laboratory6
Department of Systems Biology, Technical University of Denmark7
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark8
Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1.
We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line.
Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined.
These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Language: | English |
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Publisher: | John Wiley and Sons Inc. |
Year: | 2016 |
Pages: | 60-69 |
ISSN: | 15214141 and 00142980 |
Types: | Journal article |
DOI: | 10.1002/eji.201545890 |
ORCIDs: | Nielsen, Morten |