Journal article
Video-rate, mid-infrared hyperspectral upconversion imaging
In this work we demonstrate, to the best of our knowledge, a novel wide field-of-view upconversion system, supporting upconversion of monochromatic mid-infrared (mid-IR) images, e.g., for hyperspectral imaging (HSI). An optical parametric oscillator delivering 20 ps pulses tunable in the 2.3–4 μm range acts as a monochromatic mid-IR illumination source.
A standard CCD camera, in synchronism with the crystal rotation of the upconversion system, acquires in only 2.5 ms the upconverted mid-IR images containing 64 kpixels, thereby eliminating the need for postprocessing. This approach is generic in nature and constitutes a major simplification in realizing video-frame-rate mid-IR monochromatic imaging.
A second part of this paper includes a proof-of-principle study on esophageal tissues samples, from a tissue microarray, in the 3–4 μm wavelength range. The use of mid-IR HSI for investigation of esophageal cancers is particularly promising as it allows for a much faster and possibly more observer-independent workflow than state-of-the-art histology.
Comparing histologically stained sections evaluated by a pathologist to images obtained by either Fourier transform IR or upconversion HSI based on machine learning shows great promise for further work pointing towards clinical translation using the presented mid-IR HSI upconversion system.
Language: | English |
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Year: | 2019 |
Pages: | 702-708 |
ISSN: | 23342536 |
Types: | Journal article |
DOI: | 10.1364/OPTICA.6.000702 |
ORCIDs: | Tidemand-Lichtenberg, Peter , Pedersen, Christian , 0000-0002-5164-6130 , 0000-0001-5603-3731 and 0000-0003-2849-0390 |