Journal article
An estrogen-responsive plasma protein expression signature in Atlantic cod (Gadus morhua) revealed by SELDI-TOF MS
Compound-specific protein expression signatures( PESs) can be revealed by proteomic techniques. The SELDI-TOF MS approach is advantageous due to its simplicity and high-throughput capacity,however, there are concerns regarding the reproducibility of this method. The aim of this study was to define an estrogen-responsive PES in plasma of Atlantic cod (Gadus morhua) using the SELDI-TOF MS technique.
Protein expression analysis of male cod exposed to 17 b-estradiol (E2) showed that 27 plasma peaks were differentially expressed following exposure.There producibility of this result was evaluated by reanalyzing the samples six months later,and a significant change in expression was confirmed for 13 of the 27 peaks detected in the first analysis.
The performance of the reproducible E2-responsive PES, constituting these 13 peaks, was then tested on samples from juvenile cod exposed to 4-nonylphenol, North Sea oil, or North Sea oil spiked with alkylphenols. Principal component analysis revealed that nonylphenol-exposed cod could be separated from unexposed cod based on the E2-responsive PES, indicating that the PES can be used to assess estrogenic exposure of both juvenile and adult specimens of cod.
A targeted antibody-assisted SELDI-TOF MS approach was carried out in an attempt to identify the E2-responsive peaks. Results indicated that 2 peaks were fragments of the well-known biomarkers VTG and/or ZRP. In this study, the SELDI-TOF MS technology has shown its potential for defining compound-specific PESs in fish.
Nevertheless, thorough validtion of reproducibility, specificity and sensitivity of a PESis required before it can be applied in environmental monitoring.
Language: | English |
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Year: | 2011 |
Pages: | 2175-2181 |
ISSN: | 10902414 and 01476513 |
Types: | Journal article |
DOI: | 10.1016/j.ecoenv.2011.07.036 |
4-nonylphenol Animals Biomarkers Blood Proteins Environmental Monitoring Estradiol Estrogens Fish Proteins Gadus morhua Gonadal Steroid Hormones Male North Sea Petroleum Phenols Proteomics Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Water Pollutants, Chemical nonylphenol