Journal article
Enzymatic degradation of polymer covered SOPC-liposomes in relation to drug delivery
Polyethylenoxide (PEG) covered liposomes are used as lipid-based drug-delivery systems. In comparison to conventional liposomes the polymer-covered liposomes display a long circulation half-life in the blood stream. We investigate the influence of polyethyleneoxide-distearoylphosphatidylethanolamine (DSPE-PEG$-750$/) lipopolymer concentration on phospholipase A$-2$/ (PLA$-2$/) catalyzed hydrolysis of liposomes composed of stearoyloleoylphosphatidylcholine (SOPC).
The characteristic PLA$-2$/ lag-time was determined by fluorescence and the degree of lipid hydrolysis was followed by HPLC analysis. Particle size and zeta-potential were measured as a function of DSPE-PEG$-750$/ lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzyme activity, was observed with increasing concentrations of the anionic DSPE-PEG$-750$/ lipopolymer lipids.
The observed decrease in lag-time might be related to changes in the surface potential and the PLA$-2$/ lipid membrane affinity.
| Language: | English |
|---|---|
| Year: | 2001 |
| Pages: | 303-311 |
| ISSN: | 18733727 and 00018686 |
| Types: | Journal article |
| DOI: | 10.1016/s0001-8686(00)00058-0 |
| ORCIDs: | 0000-0002-6621-3784 and 0000-0002-4258-8960 |
1,2-distearoylphosphatidylethanolamine 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine Drug Delivery Systems Electrophoresis Hydrogen-Ion Concentration Hydrolysis Liposomes Membrane Potentials Particle Size Phosphatidylcholines Phosphatidylethanolamines Phospholipases A Phospholipases A2 Polyethylene Glycols Surface Properties
