Journal article
Genome-wide identification of tolerance mechanisms toward p-coumaric acid in Pseudomonas putida
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark1
Systems Environmental Microbiology, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Bacterial Cell Factory Optimization, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
iLoop, Translational Management, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 towards eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant towards three of the eleven compounds when compared to Escherichia coli.
Increased tolerance was for example found towards p-coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome-wide approach, Tn-seq. Libraries containing a large number of miniTn5-Km transposon insertion mutants were grown in the presence and absence of p-coumaric acid, and the enrichment or depletion of mutants was quantified by high-throughput sequencing.
Several genes, including the ABC transporter Ttg2ABC and the cytochrome c maturation system (ccm), were identified to play an important role in the tolerance towards p-coumaric acid of this bacterium. Most of the identified genes were involved in membrane stability, suggesting that tolerance towards p-coumaric acid is related to transport and membrane integrity.
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Language: | English |
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Publisher: | John Wiley and Sons Inc. |
Year: | 2018 |
Pages: | 762-774 |
ISSN: | 10970290 and 00063592 |
Types: | Journal article |
DOI: | 10.1002/bit.26495 |
ORCIDs: | Calero, Patricia , Jensen, Sheila I. , Bojanovič, Klara , Lennen, Rebecca , Koza, Anna and Nielsen, Alex Toftgaard |