Journal article
Interaction between structurally different heteroexopolysaccharides and β-lactoglobulin studied by solution scattering and analytical ultracentrifugation
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Enzyme and Protein Chemistry, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark4
Agriculture and Agri-Food Canada5
University of Copenhagen6
Department of Chemistry, Technical University of Denmark7
X-ray Crystallography, Department of Chemistry, Technical University of Denmark8
Amphiphilic Polymers in Biological Sensing, Department of Micro- and Nanotechnology, Technical University of Denmark9
Despite a very large number of bacterial exopolysaccharides have been reported, detailed knowledge on their molecular structures and associative interactions with proteins is lacking. Small-angle X-ray scattering, dynamic light scattering and analytical ultracentrifugation (AUC) were used to characterize the interactions of six lactic acid bacterial heteroexopolysaccharides (HePS-1-HePS-6) with β-lactoglobulin (BLG).
Compared to free HePSs, a large increase in the X-ray radius of gyration RG, maximum length L and hydrodynamic diameter dH of HePS-1-HePS-4 mixed with BLG revealed strong aggregation, the extent of which depended on the compact conformation and degree of branching of these HePSs. No significant effects were observed with HePS-5 and HePS-6.
Turbidity and AUC analyses showed that both soluble and insoluble BLG-HePS complexes were formed. The findings provide new insights into the role of molecular structures in associative interactions between HePSs and BLG which has relevance for various industrial applications.
| Language: | English |
|---|---|
| Year: | 2018 |
| Pages: | 746-754 |
| ISSN: | 18790003 and 01418130 |
| Types: | Journal article |
| DOI: | 10.1016/j.ijbiomac.2018.01.050 |
| ORCIDs: | 0000-0003-2658-414X , Harris, Pernille , Almdal, Kristoffer , Khan, Sanaullah , Peters, Günther H.J. and Svensson, Birte |
