Journal article
Surface functionalized thiol-ene waveguides for fluorescence biosensing in microfluidic devices : Miniaturization
Department of Micro- and Nanotechnology, Technical University of Denmark1
Polymer Micro & Nano Engineering, Department of Micro- and Nanotechnology, Technical University of Denmark2
ChemLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark3
Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark4
Thiol-ene polymers possess physical, optical, and chemical characteristics thatmake them ideal substrates for the fabrication of optofluidic devices. In this work, thiol-ene polymers are used to simultaneously create microfluidic channels and optical waveguides in one simple moulding step. The reactive functional groups present at the surface of the thiol-ene polymer are subsequently used for the rapid, one step, site-specific functionalization of the waveguide with biological recognition molecules.
It was found that while the bulk properties and chemical surface properties of thiol-ene materials vary considerably with variations in stoichiometric composition, their optical properties remain mostly unchanged with an average refractive index value of 1.566 ± 0.008 for thiol-ene substrates encompassing a range from 150% excess ene to 90% excess thiol.
Microfluidic chips featuring thiol-ene waveguides were fabricated from 40% excess thiol thiol-ene to ensure the presence of thiol functional groups at the surface of the waveguide. Biotin alkyne was photografted at specific locations using a photomask, directly at the interface between the microfluidic channel and the thiol-ene waveguide prior to conjugation with fluorescently labeled streptavidin.
Fluorescence excitation was achieved by launching light through the thiol-ene waveguide, revealing bright fluorescent patterns along the channel/waveguide interface
Language: | English |
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Year: | 2013 |
Pages: | 282-288 |
ISSN: | 15222683 and 01730835 |
Types: | Journal article |
DOI: | 10.1002/elps.201300271 |
ORCIDs: | 0000-0003-4459-8801 , 0000-0003-1065-1985 and Feidenhans'l, Nikolaj Agentoft |