Journal article
Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors†
Federal Institute for Materials Research and Testing, Berlin, Germany, Chemistry Department, Humboldt University Berlin, Germany, Institute of Molecular Biology and Bioinformatics, Charité University Medicine Berlin, Germany, and Physics Department, Danish Technical University, Lyngby, Denmark
The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells.
We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.
| Language: | English |
|---|---|
| Publisher: | American Chemical Society |
| Year: | 2010 |
| Pages: | 7421-7426 |
| ISSN: | 19327455 and 19327447 |
| Types: | Journal article |
| DOI: | 10.1021/jp910034z |
