Journal article
Neutrophil elastase-mediated increase in airway temperature during inflammation
University Clinic Tübingen1
Department of Systems Biology, Technical University of Denmark2
Centre International de Recherche en Infectiologie3
King's College London4
Verona University Hospital5
Ospedale Civile Maggiore, Verona6
Dresden University of Technology7
University of Naples Federico II8
Airway Research Center North9
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark10
...and 0 moreBackground How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. Methods We determined the temperature in the exhaled air of cystic fibrosis (CF) patients.
To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. Results Here we show a temperature of ~ 38 °C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise.
Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38 °C vs 30 °C revealed increased virulence traits and characteristic cell wall changes. Conclusion Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.
Language: | English |
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Year: | 2014 |
Pages: | 623-631 |
ISSN: | 18735010 and 15691993 |
Types: | Journal article |
DOI: | 10.1016/j.jcf.2014.03.004 |
ORCIDs: | 0000-0002-2758-7062 |
Adolescent Animals Body Temperature Case-Control Studies Child Cystic Fibrosis Disease Models, Animal Female Hot Temperature Humans Inflammation Leukocyte Elastase Male Mice Mice, Inbred C57BL Neutrophil elastase Pseudomonas Infections Pseudomonas aeruginosa Respiratory Tract Infections Temperature