Journal article
Host Resistance, Genomics and Population Dynamics in a Salmonella Enteritidis and Phage System
Bacteriophages represent an alternative solution to control bacterial infections. When interacting, bacteria and phage can evolve, and this relationship is described as antagonistic coevolution, a pattern that does not fit all models. In this work, the model consisted of a microcosm of Salmonella enterica serovar Enteritidis and φSan23 phage.
Samples were taken for 12 days every 48 h. Bacteria and phage samples were collected; and isolated bacteria from each time point were challenged against phages from previous, contemporary, and subsequent time points. The phage plaque tests, with the genomics analyses, showed a mutational asymmetry dynamic in favor of the bacteria instead of antagonistic coevolution.
This is important for future phage-therapy applications, so we decided to explore the population dynamics of Salmonella under different conditions: pressure of one phage, a combination of phages, and phages plus an antibiotic. The data from cultures with single and multiple phages, and antibiotics, were used to create a mathematical model exploring population and resistance dynamics of Salmonella under these treatments, suggesting a nonlethal, growth-inhibiting antibiotic may decrease resistance to phage-therapy cocktails.
These data provide a deep insight into bacterial dynamics under different conditions and serve as additional criteria to select phages and antibiotics for phage-therapy.
Language: | English |
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Publisher: | MDPI |
Year: | 2019 |
Pages: | 188 |
ISSN: | 19994915 |
Types: | Journal article |
DOI: | 10.3390/v11020188 |
ORCIDs: | 0000-0001-7795-1494 , 0000-0003-1973-7128 , 0000-0001-7015-0512 , Leekitcharoenphon, Pimlapas and Møller Aarestrup, Frank |