Journal article
Antibody Cross-Reactivity in Antivenom Research
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Tropical Pharmacology and Biotherapeutics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Network Engineering of Eukaryotic Cell factories, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
University of Cambridge5
Fred Hutchinson Cancer Research Center6
Department of Bio and Health Informatics, Technical University of Denmark7
Genomic Epidemiology, Department of Bio and Health Informatics, Technical University of Denmark8
Technical University of Denmark9
Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark10
...and 0 moreAntivenom cross-reactivity has been investigated for decades to determine which antivenoms can be used to treat snakebite envenomings from different snake species. Traditionally, the methods used for analyzing cross-reactivity have been immunodiffusion, immunoblotting, enzyme-linked immunosorbent assay (ELISA), enzymatic assays, and in vivo neutralization studies.
In recent years, new methods for determination of cross-reactivity have emerged, including surface plasmon resonance, antivenomics, and high-density peptide microarray technology. Antivenomics involves a top-down assessment of the toxin-binding capacities of antivenoms, whereas high-density peptide microarray technology may be harnessed to provide in-depth knowledge on which toxin epitopes are recognized by antivenoms.
This review provides an overview of both the classical and new methods used to investigate antivenom cross-reactivity, the advantages and disadvantages of each method, and examples of studies using the methods. A special focus is given to antivenomics and high-density peptide microarray technology as these high-throughput methods have recently been introduced in this field and may enable more detailed assessments of antivenom cross-reactivity.
Language: | English |
---|---|
Publisher: | MDPI |
Year: | 2018 |
Pages: | 393 |
ISSN: | 20726651 |
Types: | Journal article |
DOI: | 10.3390/toxins10100393 |
ORCIDs: | 0000-0003-2979-5663 , Engmark, Mikael , Laustsen, Andreas Hougaard , Ledsgaard, Line , Andersen, Mikael Rørdam and Lund, Ole |