Journal article
Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid
Department of Chemical and Biochemical Engineering, Technical University of Denmark1
Center for BioProcess Engineering, Department of Chemical and Biochemical Engineering, Technical University of Denmark2
Technical University of Denmark3
SINTEF4
PILOT PLANT, Department of Chemical and Biochemical Engineering, Technical University of Denmark5
This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media.
The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110 g/L/d of glycerol (with a productivity of 38 g/L/d and 11 g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12 h and 60 g/L feed.
The maximum feed concentration reached almost 90 g/L, leading though to an incomplete substrate degradation.
| Language: | English |
|---|---|
| Year: | 2017 |
| Pages: | 149-161 |
| ISSN: | 1873295x and 1369703x |
| Types: | Journal article |
| DOI: | 10.1016/j.bej.2017.09.012 |
| ORCIDs: | Varrone, Cristiano , Skiadas, Ioannis V and Gavala, Hariklia N. |
