Journal article
Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark
National Veterinary Institute, Technical University of Denmark1
Virology, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark2
Diagnostic & Development, Division for Diagnostics & Scientific Advice, National Veterinary Institute, Technical University of Denmark3
University of Copenhagen4
Technical University of Denmark5
Wageningen Bioveterinary Research6
Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark.
Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom.
Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.
| Language: | English |
|---|---|
| Publisher: | MDPI |
| Year: | 2018 |
| Pages: | 486 |
| ISSN: | 19994915 |
| Types: | Journal article |
| DOI: | 10.3390/v10090486 |
| ORCIDs: | Belsham, Graham J. , Rasmussen, Thomas Bruun , Lazov, Christina M. and Chriél, Mariann |
Coronavirus Europe Host restriction Microbiology Nucleotide sequencing Phylogenetic analysis Vespertilionidae
Alphacoronavirus Animals Chiroptera Cluster Analysis Coronavirus Infections Denmark Feces Genetic Variation Phylogeny QR1-502 Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology coronavirus host restriction nucleotide sequencing phylogenetic analysis
