Journal article
Identification of minimal sequences of the Rhopalosiphum padi virus 5' untranslated region required for internal initiation of protein synthesis in mammalian, plant and insect translation systems
University of Surrey1
Section of Vesicular virus diseases, Division of Virology, National Veterinary Institute, Technical University of Denmark2
Division of Virology, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Pirbright Institute5
Rhopalosiphum padi virus (RhPV) is a member of the family Dicistroviridae. The genomes of viruses in this family contain two open reading frames, each preceded by distinct internal ribosome entry site (IRES) elements. The RhPV 5' IRES is functional in mammalian, insect and plant translation systems and can form 48S initiation complexes in vitro with just the mammalian initiation factors eIF2, eIF3 and eIF1.
Large regions of the 5' untranslated region (UTR) can be deleted without affecting initiation-complex formation. The minimal sequences required for directing internal initiation in mammalian (rabbit reticulocyte lysate), plant (wheatgerm extract) and insect (Sf21 cells) translation systems have now been defined.
A fragment (nt 426–579) from the 3' portion of the 5' UTR can direct translation in each of these translation systems. In addition, a distinct region (nt 300–429) is also active. Thus, unstructured regions within the 5' UTR seem to be critical for IRES function.
| Language: | English |
|---|---|
| Year: | 2007 |
| Pages: | 1583-1588 |
| ISSN: | 14652099 and 00221317 |
| Types: | Journal article |
| DOI: | 10.1099/vir.0.82682-0 |
| ORCIDs: | Belsham, Graham |
5' Untranslated Regions 5' Untranslated Regions/genetics Animals Aphids Aphids/virology DNA, Viral DNA, Viral/genetics Insect Viruses Insect Viruses/genetics Mammals Mammals/virology Peptide Chain Termination, Translational Plants Plants/virology Plasmids Protein Biosynthesis Viral Proteins Viral Proteins/biosynthesis
