Journal article
Examining the Potential of Plasma-Assisted Pretreated Wheat Straw for Enzyme Production by Trichoderma reesei
Plasma-assisted pretreated wheat straw was investigated for cellulase and xylanase production by Trichoderma reesei fermentation. Fermentations were conducted with media containing washed and unwashed plasma-assisted pretreated wheat straw as carbon source which was sterilized by autoclavation. To account for any effects of autoclavation, a comparison was made with unsterilized media containing antibiotics.
It was found that unsterilized washed plasma-assisted pretreated wheat straw (which contained antibiotics) was best suited for the production of xylanases (110 IU ml(-1)) and cellulases (0.5 filter paper units (FPU) ml(-1)). Addition of Avicel boosted enzyme titers with the highest cellulase titers (1.5 FPU ml(-1)) found with addition of 50 % w/w Avicel and with the highest xylanase production (350 IU ml(-1)) reached in the presence of 10 % w/w Avicel.
Comparison with enzyme titers from other nonrefined feedstocks suggests that plasma pretreated wheat straw is a promising and suitable substrate for cellulase and hemicellulase production.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2012 |
Pages: | 2051-2063 |
Journal subtitle: | Part A: Enzyme Engineering and Biotechnology |
ISSN: | 15590291 and 02732289 |
Types: | Journal article |
DOI: | 10.1007/s12010-012-9631-x |
ORCIDs: | Hobley, Timothy John |
10060, Biochemistry studies - General 10802, Enzymes - General and comparative studies: coenzymes 39008, Food microbiology - General and miscellaneous 51522, Plant physiology - Chemical constituents Avicel boosted enzyme Biochemistry and Molecular Biophysics Bioprocess Engineering Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi Imperfecti or Deuteromycetes [15500] Trichoderma reesei species fermentation agent strain-Rut-C30 autoclavation laboratory techniques cellulase 9012-54-8 EC 3.2.1.4 hemicellulase 9025-56-3 plasma-assisted pretreated wheat straw synergistic effect xylanase 37278-89-0 EC 3.2.1.32