Journal article
Acclimation to extremely high ammonia levels in continuous biomethanation process and the associated microbial community dynamics
Acclimatized anaerobic communities to high ammonia levels can offer a solution to the ammonia toxicity problem in biogas reactors. In the current study, a stepwise acclimation strategy up to 10 g NH4+-N L−1, was performed in mesophilic (37 ± 1 °C) continuously stirred tank reactors. The reactors were co-digesting (20/80 based on volatile solid) cattle slurry and microalgae, a protein-rich, 3rd generation biomass.
Throughout the acclimation period, methane production was stable with more than 95% of the uninhibited yield. Next generation 16S rRNA gene sequencing revealed a dramatic microbiome change throughout the ammonia acclimation process. Clostridium ultunense, a syntrophic acetate oxidizing bacteria, increased significantly alongside with hydrogenotrophic methanogen Methanoculleus spp., indicating strong hydrogenotrophic methanogenic activity at extreme ammonia levels (>7 g NH4+-N L−1).
Overall, this study demonstrated for the first time that acclimation of methanogenic communities to extreme ammonia levels in continuous AD process is possible, by developing a specialised acclimation AD microbiome.
Language: | English |
---|---|
Year: | 2018 |
Pages: | 616-623 |
ISSN: | 18732976 and 09608524 |
Types: | Journal article |
DOI: | 10.1016/j.biortech.2017.09.148 |
ORCIDs: | Tian, Hailin , Fotidis, Ioannis , Mancini, Enrico , Treu, Laura and Angelidaki, Irini |