Conference paper
DNA microarrays immobilized on unmodified plastics in a microfluidic biochip for rapid typing of Avian Influenza Virus
Department of Micro- and Nanotechnology, Technical University of Denmark1
Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Polymers are widely used for microfluidic systems, but fabrication of microarrays on such materials often requires complicated chemical surface modifications, which hinders the integration of microarrays into microfluidic systems. In this paper, we demonstrate that UV irradiation can be used to directly immobilize poly(T)poly(C)-tagged DNA oligonucleotide probes on non-modified plastic surfaces.
This one-step, cost-effective process provides very high immobilization and hybridization efficiencies and is applicable to many different types of polymers. Using this microarray fabrication technique, a portable cyclic olefin copolymer (COC) microarray device containing eight individually addressable microfluidic channels was developed for fast identification of Avian Influenza Virus (AIV) by DNA hybridization.
This plastic biochip offers benefits of low fabrication cost and parallel processing of multiple samples, and could be used as a point-of-care device for clinical diagnostics and gene expression analysis.
| Language: | English |
|---|---|
| Publisher: | IEEE |
| Year: | 2011 |
| Pages: | 878-881 |
| Proceedings: | 16th International Solid-State Sensors, Actuators and Microsystems Conference |
| ISBN: | 145770157X , 145770157x , 9781457701573 , 1457701553 , 1457701561 , 9781457701559 and 9781457701566 |
| Types: | Conference paper |
| DOI: | 10.1109/TRANSDUCERS.2011.5969311 |
| ORCIDs: | Yi, Sun , Dufva, Martin , Bang, Dang Duong , Bu, Minqiang and Wolff, Anders |
Avian Influenza Virus COC microarray DNA DNA hybridization efficiency DNA microarray Fabrication Microfluidics Plastics Probes Substrates Surface morphology UV irradiation chemical surface modification cyclic olefin copolymer lab-on-a-chip microfluidic biochip microfluidics microorganisms poly(T)poly(C) polymers rapid typing unmodified plastic
