Conference paper
Micromechanical sensors for the measurement of biopolymer degradation
Nanoprobes Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Nanocarbon, Department of Micro- and Nanotechnology, Technical University of Denmark4
Technical University of Denmark5
Dynamic NEMS Group, NanoSystemsEngineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
We present microcantilever-based sensors for the characterization of biopolymer degradation by enzymes. Thin films of Poly(L-lactide) (PLLA) were spray-coated onto SU-8 cantilevers with well-known material properties and dimensions. The micromechanical sensors were immersed in solutions of proteinase K to investigate enzymatic degradation of PLLA.
A decrease of the resonance frequency after immersion indicated degradation of the biopolymer coating and allowed the estimation of the degradation rate at a specific enzyme concentration.
Language: | English |
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Publisher: | IEEE |
Year: | 2011 |
Pages: | 457-460 |
Proceedings: | 24th International Conference on Micro Electro Mechanical Systems |
ISBN: | 1424496322 , 1424496349 , 9781424496327 and 9781424496341 |
ISSN: | 10846999 |
Types: | Conference paper |
DOI: | 10.1109/MEMSYS.2011.5734460 |
ORCIDs: | Keller, Stephan Sylvest , Schmid, Silvan and Boisen, Anja |
Biopolymer coating Biopolymer degradation Enzymes Immersion Microcantilever-based sensors Micromechanical sensors POLY(L-lactide) thin films Poteinase K Resonance frequency SU-8 cantilevers Spray coating
Coatings Degradation Frequency measurement Gold Proteins Resonant frequency Young's modulus bioMEMS biochemistry biodegradable materials biological techniques biopolymer coating biopolymer degradation cantilevers enzymes immersion microcantilever-based sensors micromechanical sensors microsensors molecular biophysics polymer films proteinase K resonance frequency spray coating spray coatings