Book chapter · Journal article
Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus
In
Methods in Molecular Biology
—
2017, Volume 1572, pp. 55-70
Abstract
In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO2 laser cutting of Poly(methyl methacrylate) (PMMA) sheets.
Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution.
| Language: | English |
|---|---|
| Publisher: | Springer |
| Year: | 2017 |
| Pages: | 55-70 |
| Series: | Methods in Molecular Biology |
| Journal subtitle: | Methods and Protocols, Volume 2 |
| ISBN: | 1493969102 , 1493969110 , 9781493969104 and 9781493969111 |
| ISSN: | 10643745 and 19406029 |
| Types: | Book chapter and Journal article |
| DOI: | 10.1007/978-1-4939-6911-1_5 |
